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When reduced mAb was generated and isolated from reducing conditions prior to incubation with hydroxocobalamin, it remained red after buffer exchange to remove free hydroxocobalamin (a* = 39 without alkylation, a* = 56 with alkylation), whereas non-reduced BDS treated with hydroxocobalamin was yellow after buffer exchange (a* = 0)

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No significant toxicity has been identified in animal studies at the doses tested, and there is no established human side effect profile because the trials do not exist