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Evidence from in vitro and in vivo studies demonstrates that melatonin downregulates steroidogenic acute regulatory (StAR) protein and 3β-hydroxysteroid dehydrogenase expression under OS conditions, thus restraining excessive androgen synthesis (196), an effect particularly relevant in PCOS, where HA disrupts follicular development

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Nonmigrated cells were removed with cotton swabs, and migrated cells were fixed with ice-cold methanol and stained with 4′,6-diamidino-2-phenylindole (DAPI)