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immune cells are among the most GSH-dependent in the body Redox signalling — the GSH/GSSG (reduced/oxidised) ratio is a master regulator of cellular redox state, modulating transcription factors including NF-κB and Nrf2 Protein maintenance — glutathionylation protects cysteine residues in proteins from irreversible oxidation, preserving enzyme function Glutathione levels decline with age, chronic illness, toxin exposure, and oxidative stress — contributing to accelerated cellular aging, impaired detoxification, and systemic inflammation