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YFP/YFP and Ela-Kras G12D mice (6-week-old) were first treated with tamoxifen (30 mg/mL in corn oil) by oral gavage (T5648-1G, Sigma Aldrich, Overijse, Belgium) and 4-hydroxytamoxifen (0.3 mg/mL in corn oil) by subcutaneous injection (H7904-25mg, Sigma Aldrich, Overijse, Belgium) to recombine the LSL stop cassettes and allow the expression of Kras G12D and/or YFP from their respective endogenous locus

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1×10 5 cells were seeded in each well in a 96-well plate and cultured for 24 h